Use of a negative selectable marker for rapid selection of recombinant vaccinia virus
نویسندگان
چکیده
منابع مشابه
Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants.
The antibiotic G418 was shown to be an effective inhibitor of vaccinia virus replication when an appropriate concentration of it was added to cell monolayers 48 h before infection. Genetic engineering techniques were used in concert with DNA transfection protocols to construct vaccinia virus recombinants containing the neomycin resistance gene (neo) from transposon Tn5. These recombinants conta...
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Experimental genetics have been widely used to explore the biology of the malaria parasites. The rodent parasites Plasmodium berghei and less frequently P. yoelii are commonly utilised, as their complete life cycle can be reproduced in the laboratory and because they are genetically tractable via homologous recombination. However, due to the limited number of drug-selectable markers, multiple m...
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BACKGROUND Genetic manipulation of poxvirus genomes through attenuation, or insertion of therapeutic genes has led to a number of vector candidates for the treatment of a variety of human diseases. The development of recombinant poxviruses often involves the genomic insertion of a selectable marker for purification and selection purposes. The use of marker genes however inevitably results in a ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2011
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113667